Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES
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چکیده
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Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES.
The nematode Caenorhabditis elegans is an important model animal for biological research. Currently, transgenic C. elegans strains are mainly generated by injecting DNA encoding a gene of interest, in combination with a reporter gene, into the gonad. With this approach, the interpretation of negative results, such as the failure to observe reporter expression, is frequently required. Single, se...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113821